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stain nuclei  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc stain nuclei
    Stain Nuclei, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 2155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nuclei+staining+dapi/DAPI/pm41496357-126-51-53
    Average 98 stars, based on 2155 article reviews
    stain nuclei - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Staining:

    Article Title: Transcriptional Host Responses to Infection with Streptococcus suis in a Porcine Precision-Cut Lung Slice Model: Between-Strain Differences Suggest Association with Virulence Potential
    Article Snippet: The β-tubulin of the cilia was stained using mouse monoclonal anti-β-tubulin−Cy3 antibody (1:500, Sigma-Aldrich). .. The nuclei were stained using 4’,6-diamidino-2-phenylindole (DAPI) (0.5 μg/mL in PBS, Cell Signaling Technology, Beverly, MA, USA). .. Samples were finally embedded in ProLong Gold Antifade Reagent (Cell Signaling Technology).

    Article Title: Impaired K48-polyubiquitination downmodulates mouse norovirus propagation
    Article Snippet: Fixed cells were permeabilized in blocking solution (PBS with 30% FBS) supplemented with 0.1% Triton-X100 (Sigma-Alrich), saturated with the blocking solution and incubated ON at +4°C with specified primary antibodies ( ) in blocking buffer. .. The next day, the cells were incubated 1h at RT with the secondary antibodies conjugated with fluorophores ( ) and nuclei were stained with DAPI (Cell Signaling Technology). ..

    Article Title: YTHDF1 mediates N ‐methyl‐ N ‐nitrosourea‐induced gastric carcinogenesis by controlling HSPH1 translation
    Article Snippet: The blocking solution was discarded, and cells were washed thrice with PBS and incubated with primary rabbit anti‐YTHDF1 (1:100, ab252346, Abcam, Cambridge, UK) at 4°C overnight. .. Following three rapid washes, the primary antibody were recycled and cells were incubated with goat anti‐rabbit IgG‐488 (A‐11034; Thermo Fisher Scientific, Rockford, USA) at 37°C for 1 h. Finally, the nuclei were stained with 5% DAPI (Cell Signalling Technology, Danvers, MA, USA) for 5 min. .. The cells were analysed under an inverted fluorescence microscope (IX7; Olympus, Tokyo, Japan).

    Article Title: Natural Fatty Acid Guards against Brain Endothelial Cell Death and Microvascular Pathology following Ischemic Insult in the Presence of Acute Hyperglycemia
    Article Snippet: The next day, washing (with PBS) followed incubation with secondary antibodies (conjugated with Alexa 488); rabbit anti-mouse 1:1000, Invitrogen, Waltham, MA, USA; and Goat anti-rabbit (1:1000), Proteintech, respectively. .. To stain the nuclei, we used DAPI #4083; 1:500; Cell Signaling Technology (Cambridge, UK). .. For 4-HNE staining, we incubated brain slices with anti-4-hydroxynonenal (4-HNE) 1:1000 (EMD Millipore, St. Louis, MO, USA) and anti-CD31 (557355) 1:1000 (Proteintech, USA).

    Article Title: YTHDF1 mediates N-methyl-N-nitrosourea-induced gastric carcinogenesis by controlling HSPH1 translation.
    Article Snippet: The blocking solution was discarded, and cells were washed thrice with PBS and incubated with primary rabbit anti-YTHDF1 (1:100, ab252346, Abcam, Cambridge, UK) at 4 C overnight. .. Following three rapid washes, the primary antibody were recycled and cells were incubated with goat anti-rabbit IgG-488 (A11034; Thermo Fisher Scientific, Rockford, USA) at 37 C for 1 h. Finally, the nuclei were stained with 5% DAPI (Cell Signalling Technology, Danvers, MA, USA) for 5 min. .. The cells were analysed under an inverted fluorescence microscope (IX7; Olympus, Tokyo, Japan).

    Article Title: Cannflavin B ameliorates behavioural and neuronal systems alterations in adolescent rats exposed to prenatal valproic acid.
    Article Snippet: Sections were first washed in 1X Tris-Buffered Saline (TBS, 60.5 mM Tris, 87.6 mM NaCl pH 7.6), blocked for 2 h (10 % goat serum, 1 % BSA, 0.2 % Triton-X, 1X TBS), and incubated with mouse anti-Iba1 (ionized calcium-binding adaptor molecule 1 (Iba1), 1:200; Abcam, AB283319) primary antibody for 60 h at 4 oC. .. Following incubation, sections were washed in 1X TBS, blocked (5 % goat serum, 0.5 % BSA, 0.01 % Triton-X, 1X TBS), and incubated in an anti-mouse-Alexa 488 (1:200; Invitrogen) secondary antibody for 2 h. Slices were then washed, and on the last wash 0.4 μg/mL of DAPI (4’,6-diamidino-2-phenylindole) was added to stain nuclei (Cell Signaling, #4083). .. Brain slices were then mounted on slides using Prolong Gold (Thermo Fisher Scientific).

    Article Title: Cannflavin B ameliorates social and anxiety deficits and neuronal systems dysfunction in adolescent rats exposed to prenatal valproic acid
    Article Snippet: Following incubation, sections were washed in 1X TBS, blocked (5% goat serum, 0.5% BSA, 0.01% Triton-X, 1X TBS), and incubated in an anti-mouse-Alexa 488 (1:200; Invitrogen) secondary antibody for 2 hours. .. Slices were then washed, and on the last wash 0.4 μg/mL of DAPI was added to stain nuclei (Cell Signaling, #4083). .. Brain slices were then mounted on slides using Prolong Gold (Thermo Fisher Scientific).

    Article Title: Natural Fatty Acid Guards against Brain Endothelial Cell Death and Microvascular Pathology following Ischemic Insult in the Presence of Acute Hyperglycemia.
    Article Snippet: The next day, washing (with PBS) followed incubation with secondary antibodies (conjugated with Alexa 488); rabbit anti-mouse 1:1000, Invitrogen, Waltham, MA, USA; and Goat anti-rabbit (1:1000), Proteintech, respectively. .. To stain the nuclei, we used DAPI #4083; 1:500; Cell Signaling Technology (Cambridge, UK). .. For 4-HNE staining, we incubated brain slices with anti4-hydroxynonenal (4-HNE) 1:1000 (EMD Millipore, St. Louis, MO, USA) and anti-CD31 (557355) 1:1000 (Proteintech, USA).

    Incubation:

    Article Title: Impaired K48-polyubiquitination downmodulates mouse norovirus propagation
    Article Snippet: Fixed cells were permeabilized in blocking solution (PBS with 30% FBS) supplemented with 0.1% Triton-X100 (Sigma-Alrich), saturated with the blocking solution and incubated ON at +4°C with specified primary antibodies ( ) in blocking buffer. .. The next day, the cells were incubated 1h at RT with the secondary antibodies conjugated with fluorophores ( ) and nuclei were stained with DAPI (Cell Signaling Technology). ..

    Article Title: YTHDF1 mediates N ‐methyl‐ N ‐nitrosourea‐induced gastric carcinogenesis by controlling HSPH1 translation
    Article Snippet: The blocking solution was discarded, and cells were washed thrice with PBS and incubated with primary rabbit anti‐YTHDF1 (1:100, ab252346, Abcam, Cambridge, UK) at 4°C overnight. .. Following three rapid washes, the primary antibody were recycled and cells were incubated with goat anti‐rabbit IgG‐488 (A‐11034; Thermo Fisher Scientific, Rockford, USA) at 37°C for 1 h. Finally, the nuclei were stained with 5% DAPI (Cell Signalling Technology, Danvers, MA, USA) for 5 min. .. The cells were analysed under an inverted fluorescence microscope (IX7; Olympus, Tokyo, Japan).

    Article Title: YTHDF1 mediates N-methyl-N-nitrosourea-induced gastric carcinogenesis by controlling HSPH1 translation.
    Article Snippet: The blocking solution was discarded, and cells were washed thrice with PBS and incubated with primary rabbit anti-YTHDF1 (1:100, ab252346, Abcam, Cambridge, UK) at 4 C overnight. .. Following three rapid washes, the primary antibody were recycled and cells were incubated with goat anti-rabbit IgG-488 (A11034; Thermo Fisher Scientific, Rockford, USA) at 37 C for 1 h. Finally, the nuclei were stained with 5% DAPI (Cell Signalling Technology, Danvers, MA, USA) for 5 min. .. The cells were analysed under an inverted fluorescence microscope (IX7; Olympus, Tokyo, Japan).

    Article Title: Cannflavin B ameliorates behavioural and neuronal systems alterations in adolescent rats exposed to prenatal valproic acid.
    Article Snippet: Sections were first washed in 1X Tris-Buffered Saline (TBS, 60.5 mM Tris, 87.6 mM NaCl pH 7.6), blocked for 2 h (10 % goat serum, 1 % BSA, 0.2 % Triton-X, 1X TBS), and incubated with mouse anti-Iba1 (ionized calcium-binding adaptor molecule 1 (Iba1), 1:200; Abcam, AB283319) primary antibody for 60 h at 4 oC. .. Following incubation, sections were washed in 1X TBS, blocked (5 % goat serum, 0.5 % BSA, 0.01 % Triton-X, 1X TBS), and incubated in an anti-mouse-Alexa 488 (1:200; Invitrogen) secondary antibody for 2 h. Slices were then washed, and on the last wash 0.4 μg/mL of DAPI (4’,6-diamidino-2-phenylindole) was added to stain nuclei (Cell Signaling, #4083). .. Brain slices were then mounted on slides using Prolong Gold (Thermo Fisher Scientific).



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